首页> 外文OA文献 >A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus
【2h】

A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus

机译:一种简化的多重PCR分析法,用于快速,轻松地区分耐甲氧西林金黄色葡萄球菌中全球分布的葡萄球菌盒型染色体mec类型

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

textabstractA multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCCmec types that we tested were correctly characterized by our PCR assay. SCCmec type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type.
机译:本文开发了一种多重PCR检测方法,用于鉴定耐甲氧西林金黄色葡萄球菌(MRSA)菌株中葡萄球菌盒式染色体mec(SCCmec)的主要类型和亚型。该方法使用新颖的9价多重PCR加上两个引物对用于金黄色葡萄球菌的鉴定和耐甲氧西林的检测。我们检测到的来自马来西亚的389株临床MRSA分离株和来自Harmony采集的18株欧洲分离株具有不同的SCCmec类型,已通过我们的PCR分析正确鉴定。迄今为止,在医院和社区获得的马来西亚MRSA分离株中,SCCmec III型和V型是最常见的类型,显然出现了IVh型MRSA。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号